pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

LOCUS       pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A              12607 bp ds-DNA     circular SYN 03-MAY-2019
DEFINITION  Catalytically inactive PSPCas13b. RNase activity is inactivated by 
            mutation of catalytic histidine residues in the HEPN domains 
            (H133A/H1058A)..
ACCESSION   .
VERSION     .
KEYWORDS    pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 3, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3860
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3851
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3808..3828
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7155..7181
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7191..7271
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    7299..7887
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7352..7372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7770..7781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7890..7906)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7891..7907)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8412..8592
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8618..8635)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8624..8848
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8894..9322
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8981..9000)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9191..9212
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9331..9351)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9336..9665
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9516..9651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9578..9597
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9713..9760
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9779..10153
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10283..10404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10320..10339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10374..10393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10453..10469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10453..10469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10466..10488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10477..10493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10501..10531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10546..10567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10684..10701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10855..11443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10935..10954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11614..12474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12237..12256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12475..12579)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga cgtacggcca ccatgaacat ccccgctctg gtggaaaacc
     3901 agaagaagta ctttggcacc tacagcgtga tggccatgct gaacgctcag accgtgctgg
     3961 accacatcca gaaggtggcc gatattgagg gcgagcagaa cgagaacaac gagaatctgt
     4021 ggtttcaccc cgtgatgagc cacctgtaca acgccaagaa cggctacgac aagcagcccg
     4081 agaaaaccat gttcatcatc gagcggctgc agagctactt cccattcctg aagatcatgg
     4141 ccgagaacca gagagagtac agcaacggca agtacaagca gaaccgcgtg gaagtgaaca
     4201 gcaacgacat cttcgaggtg ctgaagcgcg ccttcggcgt gctgaagatg tacagggacc
     4261 tgaccaacgc atacaagacc tacgaggaaa agctgaacga cggctgcgag ttcctgacca
     4321 gcacagagca acctctgagc ggcatgatca acaactacta cacagtggcc ctgcggaaca
     4381 tgaacgagag atacggctac aagacagagg acctggcctt catccaggac aagcggttca
     4441 agttcgtgaa ggacgcctac ggcaagaaaa agtcccaagt gaataccgga ttcttcctga
     4501 gcctgcagga ctacaacggc gacacacaga agaagctgca cctgagcgga gtgggaatcg
     4561 ccctgctgat ctgcctgttc ctggacaagc agtacatcaa catctttctg agcaggctgc
     4621 ccatcttctc cagctacaat gcccagagcg aggaacggcg gatcatcatc agatccttcg
     4681 gcatcaacag catcaagctg cccaaggacc ggatccacag cgagaagtcc aacaagagcg
     4741 tggccatgga tatgctcaac gaagtgaagc ggtgccccga cgagctgttc acaacactgt
     4801 ctgccgagaa gcagtcccgg ttcagaatca tcagcgacga ccacaatgaa gtgctgatga
     4861 agcggagcag cgacagattc gtgcctctgc tgctgcagta tatcgattac ggcaagctgt
     4921 tcgaccacat caggttccac gtgaacatgg gcaagctgag atacctgctg aaggccgaca
     4981 agacctgcat cgacggccag accagagtca gagtgatcga gcagcccctg aacggcttcg
     5041 gcagactgga agaggccgag acaatgcgga agcaagagaa cggcaccttc ggcaacagcg
     5101 gcatccggat cagagacttc gagaacatga agcgggacga cgccaatcct gccaactatc
     5161 cctacatcgt ggacacctac acacactaca tcctggaaaa caacaaggtc gagatgttta
     5221 tcaacgacaa agaggacagc gccccactgc tgcccgtgat cgaggatgat agatacgtgg
     5281 tcaagacaat ccccagctgc cggatgagca ccctggaaat tccagccatg gccttccaca
     5341 tgtttctgtt cggcagcaag aaaaccgaga agctgatcgt ggacgtgcac aaccggtaca
     5401 agagactgtt ccaggccatg cagaaagaag aagtgaccgc cgagaatatc gccagcttcg
     5461 gaatcgccga gagcgacctg cctcagaaga tcctggatct gatcagcggc aatgcccacg
     5521 gcaaggatgt ggacgccttc atcagactga ccgtggacga catgctgacc gacaccgagc
     5581 ggagaatcaa gagattcaag gacgaccgga agtccattcg gagcgccgac aacaagatgg
     5641 gaaagagagg cttcaagcag atctccacag gcaagctggc cgacttcctg gccaaggaca
     5701 tcgtgctgtt tcagcccagc gtgaacgatg gcgagaacaa gatcaccggc ctgaactacc
     5761 ggatcatgca gagcgccatt gccgtgtacg atagcggcga cgattacgag gccaagcagc
     5821 agttcaagct gatgttcgag aaggcccggc tgatcggcaa gggcacaaca gagcctcatc
     5881 catttctgta caaggtgttc gcccgcagca tccccgccaa tgccgtcgag ttctacgagc
     5941 gctacctgat cgagcggaag ttctacctga ccggcctgtc caacgagatc aagaaaggca
     6001 acagagtgga tgtgcccttc atccggcggg accagaacaa gtggaaaaca cccgccatga
     6061 agaccctggg cagaatctac agcgaggatc tgcccgtgga actgcccaga cagatgttcg
     6121 acaatgagat caagtcccac ctgaagtccc tgccacagat ggaaggcatc gacttcaaca
     6181 atgccaacgt gacctatctg atcgccgagt acatgaagag agtgctggac gacgacttcc
     6241 agaccttcta ccagtggaac cgcaactacc ggtacatgga catgcttaag ggcgagtacg
     6301 acagaaaggg ctccctgcag cactgcttca ccagcgtgga agagagagaa ggcctctgga
     6361 aagagcgggc ctccagaaca gagcggtaca gaaagcaggc cagcaacaag atccgcagca
     6421 accggcagat gagaaacgcc agcagcgaag agatcgagac aatcctggat aagcggctga
     6481 gcaacagccg gaacgagtac cagaaaagcg agaaagtgat ccggcgctac agagtgcagg
     6541 atgccctgct gtttctgctg gccaaaaaga ccctgaccga actggccgat ttcgacggcg
     6601 agaggttcaa actgaaagaa atcatgcccg acgccgagaa gggaatcctg agcgagatca
     6661 tgcccatgag cttcaccttc gagaaaggcg gcaagaagta caccatcacc agcgagggca
     6721 tgaagctgaa gaactacggc gacttctttg tgctggctag cgacaagagg atcggcaacc
     6781 tgctggaact cgtgggcagc gacatcgtgt ccaaagagga tatcatggaa gagttcaaca
     6841 aatacgacca gtgcaggccc gagatcagct ccatcgtgtt caacctggaa aagtgggcct
     6901 tcgacacata ccccgagctg tctgccagag tggaccggga agagaaggtg gacttcaaga
     6961 gcatcctgaa aatcctgctg aacaacaaga acatcaacaa agagcagagc gacatcctgc
     7021 ggaagatccg gaacgccttc gatgcaaaca attaccccga caaaggcgtg gtggaaatca
     7081 aggccctgcc tgagatcgcc atgagcatca agaaggcctt tggggagtac gccatcatga
     7141 agggatccct tcaactgcct ccacttgaaa gactgacact gggtagttcc tacccatacg
     7201 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     7261 ccgactatgc ctaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     7321 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7381 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7441 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7501 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7561 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7621 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7681 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7741 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7801 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7861 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     7921 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7981 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8041 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8101 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8161 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     8221 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     8281 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8341 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     8401 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8461 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8521 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8581 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8881 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8941 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9241 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9301 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     9361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9481 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9601 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9661 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9721 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9781 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9841 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9901 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9961 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
    10021 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
    10081 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
    10141 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    10201 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10261 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    10321 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10381 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    10441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    11761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12601 acctgac
//